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- -- card: 10961 from stack: in.1
- -- bmap block id: 0
- -- flags: 4000
- -- background id: 3161
- -- name:
- ----- HyperTalk script -----
- on openCard
- set the lockText of field "BufferInfo" to true
- set the visible of field "helptext" to false
- put line 1 of field BufferInfo into field Name
- end openCard
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- -- part contents for background part 30
- ----- text -----
- Hepes
- pKa is 7.59
- dpK/dT is -0.014
- charge on acid species is 0
- made from Hepes_acid
- Mol.wt. is 238.31
- Shortname is Hepes
- Notes: Don't use Hepes sodium salt!!
-
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- -- part contents for background part 11
- ----- text -----
- Hepes
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- -- part contents for background part 14
- ----- text -----
- .02
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- -- part contents for background part 13
- ----- text -----
- .1
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- -- part contents for background part 12
- ----- text -----
- 7.4
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- -- part contents for background part 43
- ----- text -----
- 1000
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- -- part contents for background part 15
- ----- text -----
- 20
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- -- part contents for background part 59
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- 25
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- -- part contents for background part 10
- ----- text -----
- Buffer recipe Friday, October 27, 1989
-
- Buffer : .02M Hepes pH 7.4 at T=20 °C
- pH: 7.330 at lab temperature of 25 °C
-
-
- pH: 7.4
- T: 20°C
- pKa: 7.59, pKa': 7.548 (dpKa/dT: -0.014)
- I: .1 (Maintained with 0.092M NaCl to I= 0.092)
- Notes: Don't use Hepes sodium salt!!
-
- 1. Dissolve 4.766g Hepes_acid
- and 5.376g NaCl
- in ~800.000 ml double distilled water.
-
- 2. Titrate to pH 7.330 with monobasic acid or alkali.
-
- 3. Make up to 1000mls
-
-